Occurrence of L-Serine (L-Threonine) Deaminating Enzyme in Filamentous Fungi

Production of L-asparaginase by filamentous fungi.

L-asparaginase production was investigated in the filamentous fungi Aspergillus tamarii and Aspergillus terreus. The fungi were cultivated in medium containing different nitrogen sources. A. terreus showed the highest L-asparaginase (activity) production level (58 U/L) when cultivated in a 2% proline medium. Both fungi presented the lowest level of L-asparaginase production in the presence of g...

Novel Enzyme Family Found in Filamentous Fungi Catalyzing trans-4-Hydroxylation of L-Pipecolic Acid.

Hydroxypipecolic acids are bioactive compounds widely distributed in nature and are valuable building blocks for the organic synthesis of pharmaceuticals. We have found a novel hydroxylating enzyme with activity toward L-pipecolic acid (L-Pip) in a filamentous fungus, Fusarium oxysporum c8D. The enzyme L-Pip trans-4-hydroxylase (Pip4H) of F. oxysporum (FoPip4H) belongs to the Fe(II)/α-ketogluta...

The reversible inactivation of L-threonine hydratase of sheep liver by L-serine.

The action of partially purified L-threonine dehydratase of sheep liver on L-serine has been studied. This enzyme acts on L-serlne as a substrate but is rapidly inactivated in the process, partially at pH 8.9 and more completely at pH 7.2. However, incubation at pH 8.9, with or without L-threonine, leads to a gradual restoration of up to 90% of the original activity. The addition of pyridoxal p...

Isolation and Properties of a Homogeneous Preparation of Cystathionine Synthetase-l-serine and L-threonine Dehydratase.

Selim and Greenberg (1, 2) achieved a considerable degree of purification of L-serine dehydratase (L-serine hydro-lyase (deaminating), EC 4.2.1.13) from rat liver and demonstrated that this protein preparation contained the cystathionine-synthesizing activity of the liver (L-serine hydro-lyase (adding L-homocysteine), EC 4.2.1.21). These workers (2) also observed activity of their enzyme prepar...

The fermentation of L-threonine, L-serine, L-cysteine and acrylic acid by a gram-negative coccus.